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cara makan minyak kelapa dara nufera

MV1p12 ECIS experiment using serum free media

May 10th, 2009

After 72 hours, MVR1p12 cells were ready for experiments using ECIS arrays.
400uL of serum free media with DMEM and 1% PCN/ST was exchanged with existing media and incubated for 2.5 hours.
ECIS arrays were connected and data recorded for 2 hours to establish baseline.
100uL of serum free media was removed from each ECIS well and replaced with 200uL of media+CSE+T10 to achieve concentrations according to previous experiments.

tung Uncategorized , , ,

Wound Assay using MV1p12, 10% CSE and serum free media

May 10th, 2009

MV1p12 cells were ready for wound assay with 100% confluence 72 hours after plating into 35mm plate.
2mL Serum free media using DMEM + 1% PCN/ST was exchanged into 35mm plate.
200uL of CSE was added to achieve 10% CSE concentration.
Wound simulated using 200uL pipette tip and placed under video scope for 24 hours.

tung Uncategorized , ,

myvi atau viva jimat
She even laughed at a few of his jokes with relatively little shyness.
khasiat zaitun untuk kesehatan
stokis kelapa dara catalyst
  • minyak zaitun untuk rambut
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  • rakaman cctv kemalangan di stesen
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  • proses pengolahan kelapa sawit menjadi
  • malaysia naik harga
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    kelapa dara vco catalyst
  • He paused again, but this time she just stared at him, confused. If you had any idea the restraint I was using right now, not to take you back to my bed and make love to you again, you wouldn t doubt my feelings for you ever again.
  • pembentukan bumi secara kimia
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  • jual minyak tawon asli
  • Lucas stepped up to Elsa and pulled her into his strong embrace.

    MV1p10 cells seeded 4/13/09 at 100k ready at 54 hrs

    April 15th, 2009

    Resistances as follows:

    proses pengolahan minyak bumi menjadi bensin
    perhitungan cadangan minyak bumi
    stesen minyak petronas senarai

  • kegunaan minyak kemiri untuk rambut
  • cara nak jimat minyak kereta
  • pengolahan bumi dan gas
  • kegunaan minyak zaitun untuk wajah

  • manfaat zaitun buat rambut
    kereta kancil jimat minyak

  • pengertian bumi secara umum
  • permohonan menjadi pengusaha stesen
  • data cadangan minyak indonesia
  • adakah viva jimat
  • rapid growth technology bukit
  • bayi alergi telon
    Holder A - MV1p10 @ 100k/well

    1. 1300
    2. 1231
    3. 1299
    4. 1417
    5. 1362
    6. 1251
    7. 1343
    8. 1379

    Holder B - MV1p10 @ 100k/well

    1. 1335
    2. 1568
    3. 1285
    4. 1421
    5. 1419
    6. 1495
    7. 1380
    8. N/A

    Will use serum-free DMEM with 1% pcn/strp for experiments with additives as follows, after complete removal of DMEM with FBS from ECIS wells:

    Additive 1 2 3 4 5 6 7 8
    T10 0 4 4 4 4 0 0 0
    CSE 0 0 15 50 100 15 50 100
    DMEM 500
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  • 496
    481 446 396 485 450 400

    tung ECIS , , ,

    MV1p9 cells ready for ECIS experiment at 104 hours

    April 3rd, 2009
    No comments
  • jumlah cadangan dunia
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  • Resistances for MV1p9 cells seeded on 3/30/09 at 60k cells/well are:

    Holder A

    1. 1228
    2. 1247
    3. 1265
    4. 1206
    5. 1201
    6. 1239
    7. 1242
    8. 1246

    Holder B

    1. 1241
    2. 1150
    3. 1314
    4. 1391
    5. 1293
    6. 1310
    7. 1328
    8. 35473 -Faulty electrode

    Will plate Holder A using PBS, T10, & CSE as follows:

    Additive 1
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    bahaya lintah leech oil kesehatan
    2
    3 4 5 6 7 8
    PBS 150 146 131 96 0 135 100 0
    T10 0 4 4 4 4 0 0 0
    CSE 0 0 15 50 150 minyak hitam sesuai untuk 135lc
  • His body convulsed as it tried to adjust to the new toxic invasion of power.
  • harga kemiri untuk rambut'
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  • 15
    50
  • bahaya lintah leech oil kesehatan
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  • Much to his chagrin, Linda was more than happy to agree. Lucas would go to a mortal event more than he d ever venture out into his own world probably in part to the risk to his life.
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  • Plopping it on her head, she shook a finger at Megan. Now he was only about fifteen feet above her when he inhaled her innocence again and sighed, he could not kill her to remove that rich liquid that flowed in her pure veins.
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  • tawon khasiat
    alat jimat kereta 2012
    His eyes traveled back up to 31 Ruth Ann Nordin her face.
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  • pengobatan bahaya minyak lintah leech oil
  • He smiled at her, you don t need to fear him.
  • kegunaan kemiri untuk rambut
    absorber gas vs
  • kimia kelas x bumi
  • 150
    DMEM 100 100 100 100 100 100 100 100

    Holder B will use 10% and 30% CSE in wells 6 & 7, omitting 3% CSE.

    tung ECIS , , , ,

    MV1p9 not ready at 48 hours post seeding

    April 1st, 2009

    The 35mm dish for the wound assay is ~50-60% confluent after 48 hours at a seed density of 100k cells.

    The resistances for MV1p9 at 60k per ECIS well are:

    Holder A:

    1. 1106
    2. 1111
    3. 995
    4. 1161
    5. 998
    6. 1047
    7. 1151
    8. 1097

    Holder B:

    1. 1057
    2. 1105
    3. 1115
    4. 1153
    5. 1134
    6. 1166
    7. 1190
    8. 34819 (Faulty electrode)

    tung ECIS , ,

    Method for cigarette smoke extract production

    April 1st, 2009
    1. 500ml vacuum bottle filled with 200ml of PBS was connected to suction apparatus with suction catheter below level of PBS.
    2. Using a suction catheter connected to a 1000ml pipette, 20 unfiltered research cigarettes (1 pack) were lit and “smoked” using the vacuum port at a burn rate of ~2minutes/cigarette.
    3. The optical density of the resulting PBS mixture was 2.175 at 260nm wavelength

    tung Methods

    HC14 sample obtained from normal healthy volunteer

    March 30th, 2009
    1. ~16ml of blood obtained from normal healthy volunteer (M.B.)
    2. Samples centrifuged at 1800RCF x 25min @ 22C
    3. ~12.5ml of plasma aspirated and stored at -80C
    4. ~2.5 ml of mononuclear layer aspirated and reconstituted with PBS.  Centrifuged @ 2k RPM x 10min.
    5. Cells reconstituted with 7ml EGM-2 media and cell count was 8.2082 x 10^6 cells/ml.
    6. Cells plated onto collagen I 6well plates at 3 x 10^6 cells/well x 18 wells.

    tung EPC ,

    MV29p5 cells not ready but being monitored

    March 28th, 2009

    MV29p5 cells seeded at 60k/well on 3/26/09 are not ready and exhibit much slower growth rate than MV1 seeded at the same time.  Resistances at 48hrs are:

    1. 596
    2. 903
    3. 826
    4. 874
    5. 813
    6. 934
    7. 828
    8. 35908 (faulty electrode)

    Will monitor their resistances along with MV1 in Holder B.

    tung ECIS , , ,

    MV1p9 cells ready for treatment after 48hrs

    March 28th, 2009

    Resistances for MV1p9 cells at 60k/well (seeded 3/26/09) are:

    1. 1451
    2. 1466
    3. 1433
    4. 1474
    5. 1470
    6. 1497
    7. 1477
    8. 1508

    Will perform the same treatments as previously described using 10%, 20%, & 30% CSE with and without T10 and monitor for 40hours in Holder A.

    tung ECIS , , ,

    MV20p18 cells at 70 hours

    March 26th, 2009

    Resistances are as follows:

    Holder A-MV20p18 at 60k cells/well

    1. 1295
    2. 1272
    3. 1296
    4. 1278
    5. 1266
    6. 1260
    7. 1272
    8. 1304

    Holder B-MV20p18 @100k/well

    1. 1370
    2. 994
    3. 1324
    4. 1392
    5. 1295
    6. 1342
    7. 1354
    8. 34043-Bad well

    Starting data collection at 1130 on 3/26/09 and continue for 24 hours.

    Additive 1 2 3 4 5 6 7 8
    PBS 150 146 96 46 0 100
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    menjadi pengusaha stesen minyak

    He turned and looked over his shoulder at Elsa who screamed when she saw how drastic the change was. At that moment, she was so stunning in all her alluring innocence that his defenses shattered.
  • pemborong minyak wangi tanpa alkohol
  • keseimbangan pasaran
  • hitam untuk yamaha 135lc
  • pabrik kayu putih cap lang
    It communicated a passion that none of the others had. She slid the ring off her finger and put it on the washstand, next to the basin and pitcher of water.
  • kebaikan zaitun untuk kecantikan
  • One by one, he rotated his shoulders and his head before dropping on all fours doing two hundred pushups off his knuckles and toes to work out any of the aches he felt. That is, if it would work on something like that.
  • 50
    0
    T10 0 4 4 4 4 0 0 0
    CSE 0 0 50 100 150 50 100 150
    DMEM 100 100 100 100 khasiat minyak zaitun extra virgin
  • kelapa dara catalyst

  • khasiat minyak zaitun pada kulit
  • kimia sma kelas x minyak bumi
  • manfaat zaitun untuk kecantikan
  • makalah proses pengolahan minyak kelapa sawit
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  • mendeskripsikan proses pembentukan bumi dan gas alam
  • manfaat zaitun untuk wajah
  • proses pengolahan minyak bumi dan gas alam
    100
    100 100 100

    The table shows the amounts of each additive (in uL) for each well in Holder A. Wells 3 & 6 have 10% CSE. Wells 5 & 7 have 20% CSE. Wells 6 & 8 have 30% CSE. Wells 2-5 have 100uM of T10. All concentrations are based on a total of 500uL in each well. Holder B will omit column 6 and use 20% and 30% CSE in wells 6 & 7.

    tung ECIS , , , ,