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<doan nguyen duc pha san>
tran le xuan thich quang duc
oprah winfrey died today

trang web cua so giao duc quang nam

cong thuc lam banh duc man

het weer arnay le duc

bat binh dang gioi trong giao duc

trang web cua so giao duc quang tri

sprites opengl sdl

digidesign® user conference duc

dich can kinh hanh
ecole andre theuriet bar
no-ip duc port forwarding
trung cap nghiep vu ton duc thang

de bourgogne hotel

hinh anh toi ac phat xit duc

francois neufville duc villeroy

phat xit hoa o duc

video clip dinh duc tam

duc's restaurant maplewood mn

tuyen an nguyen duc nghia ngay 11 11

sol duc river valley

electoral institutions cleavage structures

thong tu 71 giao duc mam non
sol duc falls trail map
le moulin du duc 29

trang chu bo giao duc vn

trang web so giao duc tinh quang tri
nghi dinh 61 cp ve giao

duc de romet brut prestige champagne

nguyen duc nghia da chet cua
tam su tinh duc gia dinh

xuan le duc

tieu su doanh nhan nguyen duc kien

de dietrich quadrodens duc 500

MV1p12 ECIS experiment using serum free media

May 10th, 2009

After 72 hours, MVR1p12 cells were ready for experiments using ECIS arrays.
400uL of serum free media with DMEM and 1% PCN/ST was exchanged with existing media and incubated for 2.5 hours.
ECIS arrays were connected and data recorded for 2 hours to establish baseline.
100uL of serum free media was removed from each ECIS well and replaced with 200uL of media+CSE+T10 to achieve concentrations according to previous experiments.

tung Uncategorized , , ,

Wound Assay using MV1p12, 10% CSE and serum free media

May 10th, 2009

theatre theuriet+bar le

12 dieu y duc nganh y

dai hoc ton duc thang phong dao tao
thanh duc que me

nguyen nghia khang cao

daihoc ton duc thang nha trang

stundenbuch des duc de berry wikipedia

nhac si nguyen duc cuong sinh nam
hotel moulin du duc moelan sur mer
truong nguyen khuyen co so 4 thu duc

cong van so 5842 cua bo giao duc

cach lam banh duc nhan man
bui minh duc md
truyen thang gu o nha tho duc ba paris

le duc bakery in houston tx

thich quang duc actual video

minh-duc nguyen seattle
chau dich ton nong duc manh

xe viet duc rach gia

nguyen duc nghia duoc an xa

ban do pho nguyen quy duc

trung tam giao duc quoc phong hue
truong dh ton duc thang ca mau

chua dieu duc o hue

duong xa uot mua cua huy
chuan giao duc cho tre 5 tuoi

guillaume le batard duc de normandie

quan he tinh duc truoc hon nhan

chua hai duc canada

guillaume longue epee duc de normandie
de an 322 bo giao duc dao tao
phan tich ve 12 dieu y duc
que porta o no-ip duc usa
sol hot springs washington camping
le duc tho pronunciation

bs le duc xuan

le de richelieu paris 12
banh duc tom thit

auchan bar le duc 55000

tin moi nhat ve nguyen duc nghia 2011

10 day forecast duc washington

cinema bar le duc 55

duc de richelieu dennis wheatley

nghe si nguyen duc dat

auchan bar le duc 55

bo giao va dao tao tai tphcm

dien dan giao quoc phong ttvnol

magasin leclerc de bar le duc

truong thpt kham duc.quang nam

mairie de bar le 55

dien vien ma canh dao tran duc dung

MV1p12 cells were ready for wound assay with 100% confluence 72 hours after plating into 35mm plate.
2mL Serum free media using DMEM + 1% PCN/ST was exchanged into 35mm plate.
200uL of CSE was added to achieve 10% CSE concentration.
Wound simulated using 200uL pipette tip and placed under video scope for 24 hours.

tung Uncategorized , ,

MV1p10 cells seeded 4/13/09 at 100k ready at 54 hrs

April 15th, 2009

Resistances as follows:

Holder A - MV1p10 @ 100k/well

  1. 1300
  2. 1231
  3. 1299
  4. 1417
  5. 1362
  6. 1251
  7. 1343
  8. 1379

Holder B - MV1p10 @ 100k/well

  1. 1335
  2. 1568
  3. 1285
  4. 1421
  5. 1419
  6. 1495
  7. 1380
  8. N/A

Will use serum-free DMEM with 1% pcn/strp for experiments with additives as follows, after complete removal of DMEM with FBS from ECIS wells:

Additive 1 2 3 4 5 6 7
starr kalahiki duc's bistro

nv2 dh ton duc thang

nuong chieu duc tuan

dr. quan duc minh

quan he tinh duc tuoi 16

nguyen duc nghia tu hinh hay chua

cty cp tong ly tam thu duc long

tap duc cho bung nho video

dich thuat duc viet

may les tres riches heures du duc berry

xe viet duc di rach gia

campgrounds near sol duc hot springs

tt giao quoc phong thai nguyen
grand duc jean luxembourg

giao duc quoc phong lop 11 bai 3

nguyen thi anh linh duc trong
francois duc rochefoucauld quotes french

truong dai hoc ton thang nha trang

may from les tres riches heures de duc de berry

cinéma bar le duc le colisée tarif

le duc bakery in houston

laird room dreyfus university center

dan bau pham thanh mp3

nguyen duc nghia trong trai giam

48 loi nguyen cua duc di da phat

pham duc huyen yen

de bourgogne chatterie

48 loi nguyen cua duc phat a di da mp3

xem vieo quan he tinh duc

download no-ip duc v3.0.4
ton duc thang phong dao tao
daphne duc julia quinn
khuong duc chau kieng
8
T10

dai duc thich phap hoa 2012

bo giao duc va dao tao hai phong
banh duc man nuoc dua
louis philippe ii duc d'orleans

mairie de bar le duc 55000

belles heures duc berry met

chuyen tinh duc 9x

arnay le cross

arnay le duc 21 gite

nguyen duc anh khoa

nivea hang xach tay duc

sieu thi coopmart thu duc

trang web giao duc tinh quang nam
jean d'orléans duc de vendome
phim hoat hinh thang gu nha tho duc ba
modern history sourcebook duc de saint-simon
nguyen duc nghia bi tu hinh ngay bao nhieu
sol duc river map washington
duc de bourgogne louis xiv

48 loi nguyen duc phat di da

tran dau giua duc va y
banh duc man video

saint simon wikipedia

duc beaufort pere louis xiv

dinh duc tam hung bien vi sao phai cuoi

sol duc falls washington state

giao xu duc me fatima

chu nghia phat xit duc la gi

betimes realty group llc duc lam

sol duc campground directions

tieu su cuoc doi doan nguyen
dh ton duc thang nha trang

chu nghia phat xit thang o duc

clip cua dinh tam
truong dai hoc ton duc thang nha trang
doanh nhan nguyen thi minh duc
giao duc quoc phong 12 bai 2

dang hoa me

salmonella outbreak in united states 2012

bo giao duc va dao tao tp hcm

thon xuan duc xa yen thuong gia lam

horaire tub bar le duc ligne 1

dan vien bien duc

chua thien minh thu duc
ly do nguyen nghia giet ban gai
truong minh duc quan 1

duong xa uot mua duc huy mp3

buffet hoang yen duong ngo duc ke
teak tuinmeubelen bois le duc

truong ispace o thu

phim thang gu nha tho duc ba pari
dai su quan duc viet nam

grand duc guillaume ii luxembourg

dinh tam vs minh nhi

cho tinh yeu quay ve duc tuan

tinh dau chua nguoi anh duc

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0
4 4 4 4 0 0 0
CSE 0 0 15 50 100 15 50 100
DMEM 500 496 481 446 396 485
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arnay le duc france

sieu thi dien may nguyen kim thu duc

pham duc thanh long me

dia chi tu vien quang duc

thanh thuy duc thinh 2011

banh duc man banh bot cu nang

lich thi dau bong da hang 2

truong bo binh thu

tu vien quang duc australia

quang duc south vietnam

francois duc de la rochefoucauld feu

quy hoach treo quan thu duc

truong su pham duc thao tphcm

long me dan bau pham duc thanh

bach khoa computer thu duc

dau doan euro 2012 duc va italia

bong may doi toi-duc tri

tram nam khong quen thinh beat
test xe o thu duc

duc khiem nguyen accountant

trung cap ton duc thang nha trang

stundenbuch des duc de berry september

dai duc thich thien thuan bong may

gia dinh ong le duc thuy

francois duc de la rochefoucauld biography

sol duc hot springs wa.state

duc des lombards wikipedia

binh luan bong da duc hom nay
memoirs de saint simon
chua duc vien buddhist temple
450
400

tung ECIS , , ,

MV1p9 cells ready for ECIS experiment at 104 hours

April 3rd, 2009
No comments
chua hai duc jacksonville
nguyen duc quang economics
bieu tuong phat xit duc
dien vien hong kong tran duc dung

hang duc xach tay 100 tu a den z

sol duc hot springs reviews
web cua b o giao duc tphcm
tieu su cua doan nguyen duc
quang duc burning of a monk
nghi dinh 116 ve giao duc

daihoc ton duc thang nha trang

sol duc hot springs hours
thich quang quotes

12 dieu y duc cua do nguyen phuong

trang web bo giao duc dao tao tphcm

le duc tho la ai

les belles heures du duc de berry

tin moi nhat cua nguyen duc nghia

10 facts le duc tho
perspective mathématique grand duc

francois duc de la rochefoucauld biography

sol duc falls washington

louis d'orléans duc de

tinh buon xa vang duc tuan
toa tuyen nguyen duc nghia
ly lich ong le duc thuy

viollet le duc

sol duc falls washington state

Resistances for MV1p9 cells seeded on 3/30/09 at 60k cells/well are:

Holder A

  1. 1228
  2. 1247
  3. 1265
  4. 1206
  5. 1201
  6. 1239
  7. 1242
  8. 1246

Holder B

  1. 1241
  2. 1150
  3. 1314
  4. 1391
  5. 1293
  6. 1310
  7. 1328
  8. 35473 -Faulty electrode

Will plate Holder A using PBS, T10, & CSE as follows:

Additive 1 2 3 4 5 6 7 8
PBS 150 146 131 96 0 135 100 0
T10 0 4 4 4 4 0 0 0
CSE 0 0 15 50 150 15 50 150
DMEM 100 100 100 100 100 100 100 100

Holder B will use 10% and 30% CSE in wells 6 & 7, omitting 3% CSE.

tung ECIS , , , ,

MV1p9 not ready at 48 hours post seeding

April 1st, 2009

The 35mm dish for the wound assay is ~50-60% confluent after 48 hours at a seed density of 100k cells.

The resistances for MV1p9 at 60k per ECIS well are:

Holder A:

  1. 1106
  2. 1111
  3. 995
  4. 1161
  5. 998
  6. 1047
  7. 1151
  8. 1097

Holder B:

  1. 1057
  2. 1105
  3. 1115
  4. 1153
  5. 1134
  6. 1166
  7. 1190
  8. 34819 (Faulty electrode)

tung ECIS , ,

Method for cigarette smoke extract production

April 1st, 2009
  1. 500ml vacuum bottle filled with 200ml of PBS was connected to suction apparatus with suction catheter below level of PBS.
  2. Using a suction catheter connected to a 1000ml pipette, 20 unfiltered research cigarettes (1 pack) were lit and “smoked” using the vacuum port at a burn rate of ~2minutes/cigarette.
  3. The optical density of the resulting PBS mixture was 2.175 at 260nm wavelength

tung Methods

HC14 sample obtained from normal healthy volunteer

March 30th, 2009
  1. ~16ml of blood obtained from normal healthy volunteer (M.B.)
  2. Samples centrifuged at 1800RCF x 25min @ 22C
  3. ~12.5ml of plasma aspirated and stored at -80C
  4. ~2.5 ml of mononuclear layer aspirated and reconstituted with PBS.  Centrifuged @ 2k RPM x 10min.
  5. Cells reconstituted with 7ml EGM-2 media and cell count was 8.2082 x 10^6 cells/ml.
  6. Cells plated onto collagen I 6well plates at 3 x 10^6 cells/well x 18 wells.

tung EPC ,

MV29p5 cells not ready but being monitored

March 28th, 2009

MV29p5 cells seeded at 60k/well on 3/26/09 are not ready and exhibit much slower growth rate than MV1 seeded at the same time.  Resistances at 48hrs are:

  1. 596
  2. 903
  3. 826
  4. 874
  5. 813
  6. 934
  7. 828
  8. 35908 (faulty electrode)

Will monitor their resistances along with MV1 in Holder B.

tung ECIS , , ,

MV1p9 cells ready for treatment after 48hrs

March 28th, 2009

Resistances for MV1p9 cells at 60k/well (seeded 3/26/09) are:

  1. 1451
  2. 1466
  3. 1433
  4. 1474
  5. 1470
  6. 1497
  7. 1477
  8. 1508

Will perform the same treatments as previously described using 10%, 20%, & 30% CSE with and without T10 and monitor for 40hours in Holder A.

tung ECIS , , ,

MV20p18 cells at 70 hours

March 26th, 2009

Resistances are as follows:

Holder A-MV20p18 at 60k cells/well

  1. 1295
  2. 1272
  3. 1296
  4. 1278
  5. 1266
  6. 1260
  7. 1272
  8. 1304

Holder B-MV20p18 @100k/well

  1. 1370
  2. 994
  3. 1324
  4. 1392
  5. 1295
  6. 1342
  7. 1354
  8. 34043-Bad well

Starting data collection at 1130 on 3/26/09 and continue for 24 hours.

Additive 1 2 3 4 5 6 7 8
PBS 150 146 96 46 0 100 50 0
T10 0 4
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forum giao duc quoc phong ttvnol
bo giao duc dao tao tien giang
pham duc thanh+dan bau+lossless

www.golfclub bois le duc.nl

lich su dang bo binh thu duc
giao xu duc me fatima
memoirs duc saint simon pdf

bang xep hang bong da duc

doanh nhan nguyen duc luong

sol duc hot springs waterfall

duc de bourgogne bruges

phim tai lieu chien tranh phat xit duc

mairie de bar le duc meuse

duong xa uot mua cua duc huy

nhung dieu ran cua duc phat

truong the duc the thao thieu nien 10-10

truong anh ngu uc chau thu duc

tin tuc giao duc online

vu an xac chet khong dau do nguyen duc nghia

chez camille à arnay le duc

phim han quoc quan he tinh duc

thich quang duc buddhist monk

no ip v3.0.4

download album pham duc thanh

dai hoc ton thang wikipedia

duc thanh le md
moet.edu.vn bo giao duc

mai diem phuong luu duc hoa

48 quan he tinh duc
truong dai hoc y duc hue
4
4 4 0 0 0
CSE 0 0 50 100 150 50 100 150
DMEM 100 100 100 100 100 100 100 100

The table shows the amounts of each additive (in uL) for each well in Holder A. Wells 3 & 6 have 10% CSE. Wells 5 & 7 have 20% CSE. Wells 6 & 8 have 30% CSE. Wells 2-5 have 100uM of T10. All concentrations are based on a total of 500uL in each well. Holder B will omit column 6 and use 20% and 30% CSE in wells 6 & 7.

tung ECIS , , , ,